Saccharomyces cerevisiae cell cycle.

INTRODUCTION ......................................... 164 SYNCHRONOUS CULTURES .................. ....................... 165 Assay of Landmarks ......... ................................ 165 Induction Synchrony .......... ............................... 166 Selection Synchrony 167 CELL DIVISION CYCLE MUTANTS ......................................... 167 Diagnostic Landmarks and Terminal Phenotypes ............... ................ 167 First Cycle Arrest and Execution Points.168 The Mutant Collection .......... ............................... 171 Role of cdc Genes in the Life Cycle 171 COMPONENT PROCESSES OF THE CELL CYCLE .............. ................ 171 Bud Emergence ......................................... 171 Bud Growth ......................................... 173 Cytokinesis and Cell Separation ......................................... 173 Nuclear Structure ............................ 174 Spindle Plaque Cycle ............................ 176 Nuclear DNA Components ............................ 176 Initiation of Nuclear DNA Synthesis ........ .................... 178 Nuclear DNA Synthesis ............................ 179 Mitochondrial DNA ............................ 180 Two-Micron Circular DNA ............................ 181 Macromolecule Synthesis 182 HOW ARE THE EVENTS OF THE CELL CYCLE COORDINATED? ...... ........ 183 Dependent Pathways of Landmarks ............................................. 183 Dependent Pathways of Gene Expression 184 HOW IS CELL DIVISION CONTROLLED? ...................................... 186 Transition from Mitosis to Conjugation ......................................... 186 Transition from Mitosis to Stationary Phase .................................... 188 Transition from Mitosis to Meiosis ........................................... 188 HOW IS GROWTH INTEGRATED WITH DIVISION? ........... ................. 190 THE CONCEPT OF "START" ........................................... 191